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GST-fusion proteins are generally eluted from glutathione agarose beads with the use of excess glutathione

The deconvoluted spectrum is reported on the Certificate of Analysis alongside the HPLC data, so the 16-residue chain and the N-terminal tyrosine substitution can both be confirmed against expected values before reconstitution

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doi: 10.3389/fmicb.2017.00095 136 SenderR.FuchsS.MiloR